MICR304 IMMUNOLOGY AND SEROLOGY, SCHEDULE SPRING 2004

MICR 4600 Theoretical and Applied Immunology Spring 2023

Porter Zoom room: https://calstatela.zoom.us/j/3233436353?pwd=dGd5T3BsMUFBTFl3OU5DL0p0R2xHZz09, Meeting ID: 323 343

6353, Passcode: 144198

Prerequisites:

• [MICR 3100 or (BIOL 3401 and BIOL 3900)] and [(CHEM 2200 and CHEM 2201) or (CHEM 2201 and CHEM 4310)]; each with

a grade of C or better; or instructor consent.

CANVAS:

• The course will be administered through CANVAS using section MICR 4600-01 for all lecture and lab sections.

• All course related materials for lecture and labs will be accessible through CANVAS.

• Enrolled students automatically have access to the course web site in CANVAS through their myCSULA portal.

• You can access the Canvas Student Guide by clicking the Help icon on the left side of the CANVAS page or by going to

https://community.canvaslms.com/docs/DOC-10701

. Contact your instructor if you still have trouble accessing CANVAS.

Email account:

• You must have a CalState LA email account to access CANVAS and receive course related information. To contact your

instructors, you must use your CalState LA email account.

Textbooks:

• Lecture: Janeway’s Immunobiology; 10

Edition by Murphy, Weaver, and Berg. Publisher: Norton; ISBN: 978-0-393-88487-6

(ebook) or ISBN: 978-0-393-88489-0 (hard copy). While the 10t

edition is preferred you can also work with the 9

edition

ISBN: 9780815345053. The 9

edition textbook is available at the University Bookstore. Cheggs, Amazon, and Knetbooks,

among others, offer alterative affordable rent solutions. Free online immunology materials can be accessed through

merlotx.org.

• Laboratory: The laboratory manual by Dr. Edith Porter is available as PDF file on the course web page. Print it (no need for

color printing) and place the pages in a dedicated binder that can be disinfected or let it bind (spiral binder with hard plastic

cover pages that can be disinfected is recommended). The manual will also serve as your laboratory notebook and will be

graded at the end of the course.

Student learning outcomes: Upon successful completion of this course, you will be able to:

• define the key players of the immune response and explain how the response is orchestrated

• apply this knowledge in understanding diseases arising from a dysfunctional immune system

• describe in general a biomedical laboratory environment

• explain fundamental techniques used in clinical immunology

• describe how immunology can be used as a tool in research settings

• better record, analyze, interpret, and disseminate scientific data

• better formulate questions that lead to deeper understanding

Attendance:

• Lecture and laboratory attendance is mandatory and required for meeting the course outcomes and passing the class.

Section

Class #

Day

Time

Room

Instructor

Contact

Student Hours

Lecture 01

32274

9:25 - 10:40 AM

SH C366

Dr. Edith Porter

eporter@calstatela.edu

Tel (323) 343 6353

TR 8:30 – 9 AM

ASCL 355 or Zoom

Lab 02

32275

10:55 AM- 1:25 PM

ASCL 229

Dr. Michael Chen

mchen@calstatela.edu

Tel (323) 343 2061

M 9:30-10:30 AM

BIOS 235

Performance evaluation: 1000 points total

Lecture: 600 points

Pre- and post-course reflective essays (2 worth 10 points each)

Active learning

100

Case studies (teamwork; 5 worth 10 points each, 1 final case study worth 50 points)

Seminar synopsis and research question

150

100

150

Weekly post-lecture quizzes (15 worth 10 points each)

Midterm

Final comprehensive examination

You will write two reflective essays, one at the very beginning of the class, and one at the end of the class. Instructions for these

essays will be posted on CANVAS. These essays aim to help you connect with the class and establish early on significance of the

class content to you. Active learning exercises will consist of various group and individual activities and will be incorporated in

the lecture sessions. The points assigned for active learning exercises are for participation. Case studies will help you to apply

your theoretical knowledge to the clinical settings. The cases will be assigned to teams. Detailed instructions for the case studies

including resources will be posted on CANVAS. Asking questions is the beginning of research and advancement of knowledge

occurs through the process of finding answers to these questions. This course aims to improve your ability to ask research

questions and draws from Santana, L., & Rothstein, D. (2011). “Make just one change: Teach students to ask their own

questions. Harvard Education Press: Cambridge, MA”. We will first learn how to ask questions in general and then apply the

newly gained skill to a research seminar given by our Guest Speaker Dr. Pablo Penaloza-MacMaster, Ph.D., North Western

University. A synopsis will be prepared based on the seminar presented by Dr. Penaloza-MacMaster. You will summarize the

presentation, critically evaluate the talk and its content, briefly discuss how this relates to our class, and then present your own

unique research questions ignited by the seminar. To help you process the lecture material in a timely fashion, there will be

weekly CANVAS quizzes covering the lectures from the preceding week. The first week’s quiz will include some questions about

the syllabus. The quizzes will open Thursday evenings, allow for two attempts (with the average of the scores counting), and will

close Sunday nights at 11:55 pm. Midterm and final examination will include 10 points for a brief report on a current news

article (published during the term of this semester) in the general news (newspapers, web etc.) that relates to immunology,

either in health and disease or as a tool for diagnostic or research. An article from a science journal is not appropriate. The one-

page typed news report will be in the following format: indicate title and author(s), the source and date of publication; a

summary of the article in your own words (~ ½ page), a short description of what captured your attention/why you chose this

article, and a brief discussion how the selected article specifically relates to immunology and this class. You will upload the

completed report along with link to the news article and a copy of the actual text of the article pasted after your summary (all in

one file). Midterm and final lecture exams will consist of multiple-choice questions and short answers, drawings, experimental

data analysis, and applications of the question formulation technique.

Laboratory: 400 points

Weekly CANVAS prelab quizzes (12 worth 5 points each)

Safety quiz

Quiz 1 and 2 (40 pts each)

3 Graphs (10 pts each, due 1 week after class discussion of the experiment)

Lab notebook

Poster session (Poster 30 [team], Poster presentation 10 [individual], and Poster evaluations 10 [individual])

120

Final comprehensive examination

We will handle human blood products and body fluids that have been self-collected or purchased. Thus, an individual general

release form must be signed to participate in all laboratory exercise. To ensure proper preparation for the lab exercises weekly

pre-lab quizzes will be administered through CANVAS. These multiple-choice quizzes are based on the posted laboratory manual

and have two attempts with the average score counting. They will open Thursday evenings and close Mondays at 11 am. Face to

face quizzes and the final exam will include but are not limited to questions with short answer prompts, fill-in tables, simple

calculations, data analysis, brief hand-graphing, and interpretation of graphs. Detailed instructions for graphs, poster, and

notebook are included in the lab manual and will be reiterated in class.

Grades: Based on the total points accumulated you can earn:

Max 1000 points

B+: ≥ 86 % (860pts)

C+: ≥ 76 % (760 pts)

D+: ≥ 66 % (660 pts)

A : ≥ 92 % (920 pts)

B : ≥ 82 % (820 pts)

C : ≥ 72 % (720 pts)

D : ≥ 63 % (630 pts)

A- : ≥ 89 % (890 pts)

B- : ≥ 79 % (790 pts)

C- : ≥ 69 % (690 pts)

D- : ≥ 60 % (600 pts)

F : < 60 % (< 600 pts)

Class participation and laboratory performance will be considered for the final grade.

Keep track of your points:

Assignment

Max Pts

Your Pts

Lecture

Reflective essay 1

CANVAS post-lecture quizzes

150

Case study 1 (Team)

Case study 2 (Team)

Case study 3 (Team)

Case study 4 (Team)

Case study 5 (Team)

Final case study (Team)

Seminar synopsis & research questions

Midterm

100

Final comprehensive examination

150

Active learning (mostly Team)

Reflective essay 2

Lab

CANVAS pre-lab quizzes

Safety Quiz

Quiz 1

Quiz 2

Graph 1

Graph 2

Graph 3

Lab notebook

Poster session (Team)

Final comprehensive examination

120

Extra credit for course participation

TOTAL

1000

1: Assignments involving teamwork are indicated. The remaining assignments

will be individual work assignments.

Study suggestions:

• Always read the assigned chapter and study in particular the accompanying illustrations before attending lectures. Re-read

the chapter in detail after you have attended the lecture.

• You must have carefully read the laboratory manual for the experiment of the day before coming to lab. Weekly pre-lab

quizzes will test your preparedness.

• Take very good notes during class time.

• Take the required pre- quizzes and post-quizzes after you have reviewed the material

• Summarize each week what you have learned.

• Form study groups with your peers and discuss what is happening in class. Force yourself to explain the material you have

learned.

• Create your own tables and concept maps. Create multiple tables that organize the teaching material from different

viewpoints.

• Contact your instructors by email and/or come to student hours when you are unclear about the material covered.

• Utilize the resources at the writing center (http://www.calstatela.edu/centers/write_cn/

• Learn to recognize when you need help and get help in a timely manner.

• Keep track each week how many hours you are studying and in which ways. Remember that per unit 2 – 3 hours study

outside of class are required for a good grade.

• Study, study, study, study, study........

Time

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

Sunday

Lec

10a

Lec

11a

Lab

12p

Lab

10p

General Policies:

• No make-up examination/test/quiz/reports or re-teaching for missed events. Missed events will be set as “0 points” unless

satisfactorily justified (e.g. doctor’s note) according to “Missed Class Time and Makeup Policy”

(https://www.calstatela.edu/academicsenate/handbook/ch5). Assignments turned in late will not be accepted!

• The Drop/Incomplete Policy explained in the University General Catalogue will be strictly followed

(https://ecatalog.calstatela.edu/content.php?catoid=70&navoid=8154&hl=incomplete&returnto=search#ce3

• You are expected to read and abide by the University’s Academic Honesty Policy, which can be found at

http://www.calstatela.edu/academicsenate/handbook/ch5a

. Students who violate this policy will be subject to disciplinary

action, and may receive a failing grade in the course for a single violation.

• You are responsible for the prerequisites for this course and are encouraged to discuss any questions regarding the policies

and prerequisites with the instructor.

• Reasonable accommodation will be provided to any student who is registered with the Office of Students with Disabilities

and requests needed accommodation. Please contact OSD to arrange for appropriate accommodations.

• Students in Distress can seek services provided by the health center

(https://www.calstatela.edu/studenthealthcenter/caps), Dean of Students (http://www.calstatela.edu/deanofstudents),

and the University (http://www.calstatela.edu/healthwatch).

You are strongly encouraged to work with your instructors throughout the course.

Land Acknowledgement Statement:

Consistent with our values of community and diversity, we have a responsibility to acknowledge and make visible the university’s

relationship to Native peoples. By offering this Land Acknowledgement, we affirm Indigenous sovereignty and will work to hold Cal

State LA more accountable to the needs of American Indian peoples. Prepared by Dr. Kimberly Robertson (Mvskoke) and Tongva Elder

Julia Bogany, 2020, and Approved by the Cal State LA Academic Senate on April 27, 2021.

If delivered by a Tongva student, faculty or staff, or invited community member

We, the Indian people, the traditional caretakers of this landscape are the direct descendants of the first people who formed our

land, our worlds during creation time. We have always been here. Our ancestors prepared and became the landscapes and worlds for

the coming humans with order, knowledge and gifts embedded in the landscape. Our ancestors, imbued the responsibility and

obligation to our original instructions, guided by protocol and etiquette to be part of, take care of and ensure the welfare of the

extended family and community defined in its most inclusive expression, the NATURE and to pass those teachings and responsibilities

onto our children, grandchildren and many generations to come. (AND to all those that now live here.). Written by Tongva Elder Julia

Bogany, 2020

If delivered by anyone other than a Tongva student, faculty or staff, or invited community member

With great respect, Cal State LA acknowledges the Tongva people as the traditional caretakers of Tovaangar (TOE-von-gar) – the

Tongva world, including the Los Angeles Basin, South Channel Islands, San Gabriel and Pomona Valleys, and portions of Orange, San

Bernardino, and Riverside Counties. Cal State LA is located within these lands. As an institution located on unceded Tongva land, we

pay our respects to the ancestors, elders, and our relatives/relations, past, present, and emerging.

Planned Schedule (subject to change)

Week

Day

Date

Lecture

Lab

1/24

1/26

Course requirements, student assessment

Overview (Chapter 1)

History and major concepts of immunology; Cells,

organs, and microenvironments of the immune

system; Introduction to cytokines; Immunological

Techniques

Reflective essay 1 due (10 pts)

Check in, laboratory and safety rules

Notebook instructions

Microscopy (blood cells, lymphatic organs)

1/31

2/2

Question formulation exercise

Innate Immunity

Anatomical barriers (Chapter 2); Antimicrobial

peptides & lipids (Chapter 2)

Cation depletion of saliva

Lysoplate (Graph 1, 10 pts)

2/7

2/9

Complement (Chpt. 2)

Epithelial cells (Chpt. 2); Pattern recognition receptors

(Chpt. 3)

E. coli radial agar diffusion assay

Epithelial cells and normal microbiota

2/14

2/16

Phagocytes, Chemotaxis (Chpt. 2,3)

NK cells (Chpt. 3); Induced responses of innate

immunity (Chpt. 3)

Complement analysis: total hemolytic

activity (Graph 2, 10 pts)

Complement fixation reaction (PP)

Bloodborne pathogen training (PP) (Safety

Quiz 10 pts)

2/21

2/23

Adaptive Immunity

Lymphocytes, lymphocyte receptor signaling (Chpt. 7)

Antibodies I (Chpt. 4)

Phagocytosis of Candida albicans

2/28

3/2

Antibodies II (Chpt. 5)

B cells (Chpt. 8)

Quiz 1 (40 pts)

Detection of CRP antigen by latex

agglutination

Immune electrophoresis (#272)

Radial immunodiffusion (#273)

3/7

3/9

Midterm (100 pts)

T cells I (Chpt. 4, 5)

Quantitative ELISA (#278)

3/14

3/16

T cells II (Chpt. 8)

MHC complex (Chpt. 6)

SDS-PAGE (saliva)

Lysozyme immunoblot – Day 1

3/21

3/23

T cell mediated immunity I (Chpt. 9)

The humoral immune response (Chpt. 10)

Lysozyme immunoblot – Day 2

Blood typing- use your own blood

Spring break (3.27 – 4.02.2023)

4/4

4/6

Integrated dynamics of the innate and adaptive

immune response (Chpt. 11)

The mucosal immune response (Chpt. 12)

Quiz 2 (40 pts)

Detection of antinuclear auto-antibodies

4/11

4/13

Guest Seminar (Zoom): Dr. Pablo Penaloza-

MacMaster, Northwestern University (Synopsis 30 pts)

Failures of the Immune system

Immunodeficiencies I (Chpt. 13)

Introduction to Flow (PP)

Introduction to monoclonal antibodies (PP)

Lymphocyte typing for CD4 & CD8 (Graph

3, 10 pts)

Introduction to the hemocytometer

4/18

4/20

Immunodeficiencies II (Chpt. 13)

Allergies and allergic diseases (Chpt. 14)

Mononuclear cell isolation

4/25

4/27

Guest Speaker: Dr. Nandita Bose, HiberCell Inc.

Tolerance, autoimmunity, transplantation (Chpt. 15)

Poster preparation

5/2

5/4

Cancer and the immune system (Chpt. 16)

Clinical Applications of Immunology

Serology; Immunotherapies (Chpt. 16)

Poster session (50 pts)

5/9

5/11

Vaccines (Chpt. 16)

Final Case Study Presentations (50 pts)

Lab final (120 pts)

PP: PowerPoint only

Final Examination (150 pts): TBA

Reflective essay 2 (10 pts) due Friday, May 19, 2023; 11:55 pm